RNA

Part:BBa_K2611009:Design

Designed by: Yumei Yang   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA (J23100)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need to make sure that this sgRNA sequence can bind to the reverse sequence of J23100 promoter, and at the meantime, it cannot bind to any other DNA sequence of E.coli plasmids nor genome.


Source

We designed the sgRNA sequence by ourselves and had it synthesized by Tsingke Biological Technology.co.

References